1 INSTRUMENTAL HANDLING AND APPLICATIONS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract : Chromatography is described as a group of methods used to separate components in a mixture. There are two phases to this technique: stationary and movable phases. The difference in the partition coefficients of the two phases serves as the basis for the separation of constituents. The word "chromatography" comes from the Greek words "chroma" (colour) and "graphein" (to write). For measuring pharmaceutical and environmental sample quality and quantity, high performance liquid chromatography (HPLC) is a crucial technique. It is the most adaptable, secure, dependable, and quick chromatographic approach for determining the quality of medicinal ingredients. The analytical chemistry method of high-performance liquid chromatography (HPLC; formerly known as high-pressure liquid chromatography) is used to separate, recognise, and quantify each component in a mixture. One type of liquid chromatography, HPLC, uses a liquid as the mobile phase. The most used kind of HPLC is reversed-phase HPLC. In a reversed-phase system, the stationary phase is relatively non-polar while the mobile phase is relatively polar. A solvent reservoir, pump, injector, column, detector, and integrator or acquisition and display system are components of HPLC instrumentation. The column where separation takes place is the brain of the system. HPLC can be used to identify, quantify, and resolve a compound, among other pieces of information.

Keyword : Chromatography, Pressure Liquid Chromatography, Types, Instrumentation, Application, Advantages & Disadvantages of HPLC